pCW57-MCS1-P2A-MCS2 (GFP)

LOCUS       pCW57-MCS1-P2A-MCS2 (GFP)             7837 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    pCW57-MCS1-P2A-MCS2 (GFP)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1919..2233
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1998..2016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2034..2052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2070..2088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2105..2123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2141..2159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2274..2330
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        2358..2868
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2787..2805
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2886..2895
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2892..3587
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             3603..3656
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3657..4403
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4443..4463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4861..4872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5077..5310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5382..5503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5419..5438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5473..5492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5543..5678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5605..5624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5678..5749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5750..6610
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5968..5987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6781..7369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7270..7289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7523..7540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7657..7678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7693..7723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7736..7758
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7755..7771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7755..7771
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7790..7810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7792..7810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcgaatc gatatgtcga
     1921 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     1981 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2041 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2101 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2161 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagaa ttggctagcg aattcgtcga caccggttcc ggagccacga
     2281 acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct aggctgcaga
     2341 cgcgttgtac aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     2401 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     2461 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     2521 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     2581 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     2641 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     2701 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     2761 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     2821 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagca attcacccgc
     2881 gcgcggccac catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca
     2941 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcacccccg
     3001 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
     3061 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
     3121 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
     3181 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
     3241 tgatcggcga cttcaaggtg atgggcaccg gcttccccga ggacagcgtg atcttcaccg
     3301 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgatc
     3361 tggatggcag cttcacccgc accttcagcc tgcgcgacgg cggctactac agctccgtgg
     3421 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
     3481 ccatgttcgc cttccgccgc gtggaggagg atcacagcaa caccgagctg ggcatcgtgg
     3541 agtaccagca cgccttcaag accccggatg cagatgccgg tgaagaattc gaaggaagcg
     3601 gagaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     3661 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     3721 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     3781 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     3841 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     3901 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     3961 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     4021 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     4081 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     4141 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     4201 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     4261 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     4321 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     4381 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     4441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4981 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     5041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5281 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5341 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5521 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5581 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5641 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     5701 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5761 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5821 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5881 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5941 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6001 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6061 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6121 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6181 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6241 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6301 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6361 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6421 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6481 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6541 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6601 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6661 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6721 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6781 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6841 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6901 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6961 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7021 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7081 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7141 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7201 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7261 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7321 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7381 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7441 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7501 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7801 tcactaaagg gaacaaaagc tggagctgca agcttaa
//